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Real-time PCR (qPCR)

Whether you're new to real-time PCR, also called quantitative PCR (qPCR), or want to learn about new applications for qPCR, we have the learning material, including videos and webinars, to help you understand the technology and get started quickly.

qPCR combines PCR amplification and detection into a single step. This eliminates the need to detect products using gel electrophoresis, and more importantly it enables the method to be truly quantitative. With qPCR, fluorescent dyes are used to label PCR products during thermal cycling. Real-time PCR instruments measure the accumulation of fluorescent signal during the exponential phase of the reaction for fast, precise quantification of PCR products and objective data analysis.

Real-time PCR, also known as qPCR, is used for many applications, including gene expression analysis, microRNA analysis, single nucleotide polymorphism (SNP) genotyping, copy number variation (CNV) analysis, and even protein analysis. This versatile technology can be used for both relative and absolute quantification methods. Real-time PCR applications are easy to implement using our wide selection of TaqMan® Assays and a comprehensive family of real-time PCR instruments. Learn more about the applications enabled by real-time PCR below.

 
 
 
 

 

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